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BECKMAN COULTER AMPURE XP MANUAL
Note the most common ratio used for NGS library cleanup is 1.8x.ĪMPure XP is a flexible reagent suitable for manual or automated workflows. Traces show relative differences in peak height and recovery efficiency.
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Cleanups were performed using 1.8x (red), 0.9x (blue), 0.7x (green), and 0.6x (aqua) reagent to sample ratios as shown. Only AMPure XP provides predictable performance while maintaining efficient recovery proven by the relative difference between the reference and 1.8x ratio in the traces below.Īgilent TapeStation traces of sheared gDNA purified using Ampure XP, Reagent “O”, and Reagent “K”.
BECKMAN COULTER AMPURE XP SERIES
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HAECs were grown on 0.2% gelatin-coated flasks, plates, or dishes and experiments were performed at passage 9. Human aortic endothelial cells (HAECs) (Lonza, CC2535 Walkersville, MD) were cultured in medium M199 (Hyclone laboratories, Logan, UT) supplemented with 15% fetal bovine serum (FBS H圜lone) endothelial cell growth supplement (ECGS, 50μg /mL BD Biosciences, San Jose, CA) heparin (50μg/mL) and 1% penicillin, streptomycin, and amphotericin (PSA Invitrogen, Carlsbad, CA). Protocols: HAECs were collected using RNAeasy kit. Temple University Lewis Katz School of Medicine